BioSanitizer
An Effective Alternative Medical Waste Disposal Technique
Dr.Nirmala Ganla Om Prasuti Graha and Dr.Renu Bharadwaj Prof.
Microbiology Dept. BJMC Pune
Abstract
Hospitals are
socially obligated to dispose and maintain a clean environment and
to dispose of medical waste in order to prevent pollution and
infection within and near the hospital. To this end the Bio-Medical
(Waste management & Handling) rules 1998 recommend that all
infectious waste must be incinerated. Incineration though an
effective method of waste disposal is associated with various
environmental hazards which has resulted in on site incinerators
from various parts of the world from shutting down. Alternatives to
incinerators are being looked at. This study attempts to analyze the
suitability of an alternative method for dealing with hospital waste
by using Bioconversion in the form of Sujala Biosanitiser. Over a
four-year period waste from a 12 bedded maternity hospital was
treated with this product. It resulted in a 99% reduction in volume
with no aesthetic nuisance. The end product was also monitored
microbiologically for its safety. It was similar in its microbial
content to soil and no pathogens were detected. In order to be
doubly sure, we spiked samples of treated hospital waste and garden
soil with Hepatitis B positive blood and salmonella cultures. Follow
studies revealed early disappearance of the Hepatitis virus from the
treated samples. It thus seems a promising cost effective
alternative technique for handling of infectious waste.
INTRODUCTION
The effective management of medical waste poses a wide range of
unresolved queries. Medical waste disposal is associated with
health, environmental and aesthetic hazards. The Ministry of
environments and Forests, Govt. of India has issued the Bio-Medical
waste act, which issued guidelines for the management of hospital
waste in India. It makes it mandatory for all infectious waste from
hospitals to be incinerated. [1] This involves a large capital
investment, recurring cost, handling, transportation and land
filling.
We as doctors were
being blamed for not taking care of the hospital waste and
contributing to the spread of disease. The truth is, only 5% of all
hospital waste generated is infectious [2] and mere presence of
pathogens does not necessarily mean that disease is certain. Unless
the triad of host, agent and environment is complete, a person
cannot fall sick. We also know that not all infectious patients are
hospitalised. If incineration is the answer to halting the spread of
infection in society, the logical extension is to decide the mode of
disposal of a dead body by studying the cause of death. A person’s
little finger, even if not infected has to be burnt by law, but a
whole body can be buried. Only because it came out from a hospital
and not a home. So what is the purpose of incineration?
Are we aware that the
side effects of the process are so terrible that they and are going
to have far reaching damaging effects not only on our present lives
but the future generations as well?
Incinerators do not
make waste disappear, they reduce it to ash and to atmospheric
emissions. Since 1985 in U.S. more than 300 trash incinerators have
been put on hold [3]. In a study from Great Britain it was
demonstrated that the incidence of adult cancers increased upto a
radius of 7 kms around the site of the incinerator. [4]
Incinerator companies
falsely argue that modern incinerators do not generate dioxins and
furans. However, a hundred times more dioxin may leave the
incinerator on the fly ash than is emitted into the air from the
smokestacks. This consists of residues captured before going up the
smoke stacks. Also a number of toxic compounds, including dioxins
and furans, are actually created on the fly ash in a process called
post combustion formation. Ironically, this means that the better
the air pollution control, the more toxic the ash! Toxic metals are
also trapped in the fly ash. The ash from these incinerators is
disposed of in land - fills and will eventually contaminate
groundwater. The heavy metals from this ash can produce neurological
disorders in children and reproductive problems in women. Acid gases
like sulfur dioxide aggravate symptoms of lung and heart disease.
Nitrogen gases can be fatal in high concentrations and increase
susceptibility to respiratory infections.
In our hospitals
technologies like incineration fail due to unavailability of trained
personnel to run them Poor operation and maintenance result in
incinerators which not only do not destroy waste, but also require a
lot of fuel to run and are often out of order.
So, what are the
alternatives? Hydroclaving and Microwaving are finding some place in
affluent countries but do not seem financially viable solutions for
us. Non Government Organisations(NGOs) have been working to find
solutions within the community. They have highlighted the role of
the ragpicker and emphasized the separation of biodegradable from
nonbiodegradable wastes at home. NGO’s have studied and promoted
low-cost technologies such as composting and vermiculture. More
study needs to be done to ascertain their role in the management of
Medical waste.
This article offers a
low cost, safe solution to the vexed problem of medical waste. It is
based on the principle of BIOCONVERSION.
- We are pleased to
share with you our experience of treating biodegradable hospital
waste from our 12 bedded maternity home, with Dr. Bhawalkar’s Sujala
biosanitiser. This works on the principle of aerobic breakdown of
waste rather than anaerobic decomposition, which normally occurs
[5]. The present study was done to study the feasibility and
aesthetics of this biosanitiser. Microbiological studies were
undertaken to ascertain the effect of the product on decreasing the
infectious health hazards associated with medical waste The results
have been reassuring.
METHODOLOGY
I) Construction:
We
constructed two concrete bins 3ft.widex3ft in height x3ft.depth
against the compound wall. They had two openings each- on top and in
front to serve as inlet and exit respectively. The top was covered
with a wire mesh. The front had an openable door. To begin with, we
spread coconut fibre, added hospital waste, kitchen waste, sujala
powder and covered everything with dry leaves. We put in sanitary
napkins, placentae and dressings from surgeries. The system was kept
moist at all times.
II) Microbiological follow up:
In the present study 20 samples each of treated hospital waste,
untreated hospital waste and garden soil from the same area were
subjected to aerobic, anaerobic and fungal culture to determine the
microorganisms present in them.
The aerobic isolates
were identified by standard techniques described in Macky and
M’Cartney [6]. The anaerobic isolates were identified by techniques
described by Willis [7]. The fungal isolates were identified on the
basis of their macroscopic and microscopic morphology by standard
techniques [8].
The treated samples
were also examined microbiologically for the presence of ova and
cysts of parasites.All samples were examined for the presence of
Hepatitis B antigen using the Acon Biotech rapid kit. In order to
ascertain the efficacy of the treatment procedure in dealing with
infectious pathogens in the hospital environment 5 samples of
treated hospital waste and garden soil were spiked with varying
concentrations of Hepatitis B antigen and Samonella typhi and the
samples were followed up at weekly intervals to detect their
presence.
RESULTS
A) Physical
appearance and aesthetics of Hospital Waste: Over a four-year
period between Oct 97 and Oct 2001 the system has successfully
converted 15,550 sanitary napkins, 1172 placentae and dressings from
1050 surgeries to a MUDLIKE END PRODUCT. The volume reduction
has been amazing. Four bins of 50lts. capacity were converted to
half a kg.of end product. The “bioreactor “ has worked silently.
There is absolutely no nuisance of smell o r flies. 400 kgs. of
manure has been harvested and used in our farm.
B) Microbiological Study: Treated hospital waste compared favourably with garden soil
as far as its microbiological content.
The following
chart shows the various organisms detected.
A)
AEROBIC ORGANISMS |
Sr. No. |
Name Of The Isolate |
Number |
|
|
THW |
UHW |
S |
1 |
Ps. aeruginosa |
0 |
1 |
0 |
2 |
Klebsiella sp. |
2 |
12 |
0 |
3 |
E. coli |
0 |
2 |
0 |
4 |
Staphylococcus sp. |
9 |
9 |
7 |
5 |
Citrobacter sp. |
1 |
1 |
0 |
6 |
Nonfermentors |
7 |
6 |
5 |
7 |
Enterobacter sp. |
1 |
0 |
0 |
8 |
Ps. sp. other than aeruginosa |
10 |
6 |
0 |
9 |
Bacillus sp. |
19 |
6 |
6 |
|
|
|
|
|
B) ANAEROBIC ORGANISMS |
Sr. No. |
Name Of The Isolate |
Number |
|
|
THW |
UHW |
S |
1 |
Clostridium sp. |
8 |
4 |
3 |
2 |
Bacteroids sp. |
2 |
1 |
0 |
3 |
Fusobacterium sp. |
2 |
0 |
0 |
4 |
Porphyromonas sp. |
1 |
0 |
0 |
5 |
Prevotella sp. |
0 |
1 |
0 |
6 |
Other gram negative bacilli |
3 |
2 |
1 |
|
|
|
|
|
C) FUNGAL ISOLATES |
Sr. No. |
Name Of The Isolate |
Number |
|
|
THW |
UHW |
S |
1 |
Candida albicans |
0 |
2 |
0 |
2 |
Candida spp other than albicans |
0 |
1 |
1 |
3 |
Aspergillus sp. |
6 |
0 |
6 |
4 |
Gliocladium sp. |
1 |
0 |
0 |
5 |
Cephalosporium sp. |
1 |
0 |
0 |
6 |
Curvularia |
1 |
0 |
0 |
7 |
Fusarium sp. |
0 |
1 |
0 |
8 |
Phoma sp. |
0 |
0 |
1 |
9 |
Phialophora dermatitidis |
1 |
0 |
0 |
10 |
Unidentified fungii |
6 |
2 |
6 |
TWH:
Treated Waste UWH: Untreated Waste S: Soil
Aus.Ag :Absent in all the samples
Parasites:
No ova or cysts were detected
The pathogenic organisms present in untreated waste
decreased in the treated waste. The difference was statistically
significant using the chi square test. (x2=8.44, p<0.05)
The fungi
and nonpathogenic organisms were statistically similar in the
treated waste and soil.
In order to check the efficacy of the system on
major hospital pathogens i.e. Hepatitis B and salmonella, we
“spiked” samples of treated waste and garden soil with various
dilutions of hepatitis B virus and salmonella. Five samples of
each were studied over three weeks.
The results were as follows for Hepatitis B:
Table 2: Showing the presence of HBs Ag in Treated hospital waste
& garden soil
TWH: Treated Waste S: Soil
The HBsAg disappeared from the treated hospital waste by the end
of the 2nd week while it persisted in the soil till 4
weeks.
Samples were spiked with Salmonella in initial concentrations of
300million orgs./ml. per gram The results were as follows:
Salmonella were absent by 4 weeks in both treated hospital waste
and garden soil.
DISCUSSION
Biodegradable
waste has been converted successfully to an environmentally safe
end product, which has been used as manure in our garden. Dr.
Bhawalkar’s Sujala Biosanitizer was used to hasten nature’s
biodegradation process. It was an ecologically friendly procedure.
No smell, insect, or aesthetic nuisance was encountered through
out the 3 years of the study. It has been a cost-effective
technique
It has been a
one-time investment. It cost Rs. 5100 to set up the system with no
recurring costs. The Pune Municipal Corporation has now set up a
new incinerator. As a small hospital we will be paying Rs 6960/
per year and these are likely to be raised in the future.
Though various
modifications of vermiculture are being used in Pune for disposal
of household waste, they have not been tried for medical waste.
Doubts were expressed as to the safety of the end product .So the
microbiological assay of waste and end product after decomposition
was undertaken. The microbiological study has been revealing and
reassuring. Microbiological analysis of the end product has shown
that at the end of 4 weeks of treatment all pathogens were
destroyed and the microorganisms present in the end product
resembled those in the soil. If more hospitals try out this
technique and give feed back of their experience we may soon have
a simple alternatives for waste disposal, which are more suitable
for our environment.
CONCLUSIONS
We in India are at
the crossroads of making investment decisions about medical waste
technologies. It is important that we make the right choices both
in terms of cost as well as other impacts of these choices on
society at large. We cannot turn a blind eye to the hazards of
incineration. It is in the interest our future generations to
search for alternative methods for Hospital Waste disposal that
are safe for the community. The present study has evaluated an
eco-friendly method of waste disposal, which requires minimal
handling. It also involves only a one-time investment and the end
product is safe and useful.
ACKNOWLEDGEMENTS
We would like to
acknowledge the help given by Dr U.S. Bhawalkar in inspiring the
study and providing the Sujala Biosanitiser.
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